Journal: Water

Article Title: Effects of Underwater Lighting Time on the Growth of Vallisneria spinulosa Yan and Its Water Restoration Process

doi: 10.3390/w16243697

Figure Lengend Snippet: Figure 6. CLSM diagram of leaf–biofilm complex in different light duration ratios. (a) LV: V. spin- ulosa + low light duration ratio, (b) MV: V. spinulosa + medium light duration ratio, and (c) HV: V. spinulosa + high light duration ratio. Red is EPS polysaccharide stained with Texas red, green is protein stained with FITC, and bright blue is DNA stained with DAPI.

Article Snippet: CLSM Determination Plant leaves were collected and cut into small pieces of 5 mm × 5 mm and then placed in a 24−well microplate containing 2.5% glutaraldehyde (0.1 M PBS, pH = 7.2) phosphate buffer for 24 h. After washing 3–5 times with 0.1 M PBS solution (pH = 7.2) (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), the DNA, extracellular polysaccharides, and proteins were labeled with the fluorescent dyes DAPI, Con A−Texas red conjugate solution (Invitrogen, San Diego, CA, USA), and FITC (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), respectively.

Techniques: Staining

Journal: Journal of Extracellular Vesicles

Article Title: Chemotherapy‐induced release of ADAM17 bearing EV as a potential resistance mechanism in ovarian cancer

doi: 10.1002/jev2.12338

Figure Lengend Snippet: ADAM17 is proteolytically active on isolated ascites‐EV. ADAM17 proteolytic activity of ascites‐EV was quantified using a cell‐free ADAM17 activity assay. 1 × 10 9 ascites‐EV of three OvCa patients (#1, #2 and #3) or PBS as a control were incubated at 37°C for 80 min with a fluorogenic substrate. Relative fluorescence units (RFU) were measured every 30 s. RFU of the PBS control was subtracted from the RFU of the EV incubation. (a) Kinetics of ADAM17 activity in ascites‐EV, one representative of three biological replicates is shown. (b) Mean RFU (endpoint) of the cleavage product of three biological replicates + SEM is displayed. Based on the Shapiro–Wilk normality test, statistical significance was analysed by RM one‐way‐ANOVA following Tukey's multiple comparison test. * p < 0.05, ** p < 0.01.

Article Snippet: The enzymatic activity of ADAM17 on EV was analysed using a commercially available quenched fluorogenic peptide substrate (Abz‐Leu‐Ala‐Gln‐Ala‐Val‐Arg‐Ser‐ser‐Ser‐Arg‐Dap(DNP)‐NH2) (Biozol, #BYT‐ORB104351).

Techniques: Isolation, Activity Assay, Control, Incubation, Fluorescence, Comparison